assay characteristics performance somatic 1 mastr plus a molecular research assay for the identification of snvs and indels in nras , kras and braf genes associated with various types of cancer. the flexible research assay can analyze the most frequent mutations (plex 1 only) and for the entire coding regions of nras , kras and braf . application ? for detection of mutations, snvs and indels, in nras , kras and braf in ffpe-derived dna. genes analyzed: nras, kras and braf genomic region analyzed: 4.6 kb number of amplicons: 30 amplicon length: 168 C 255 bp number of plexes: 3 design compatible with illumina nextseq, miniseq and miseq systems iontorrent ngs systems number of samples/run* @ 5 % vaf sample : miseq v2: 70 miseq v3: 129 uniformity of amplifcation (0.2x mean coverage) > 97.5 % on target read counts > 99 % * number of samples per run for illumina & iontorrent ngs systems can be calculated via the sequencing calculator .
workflow plan run multiplex pcr quality control universal pcr quality control equimolar pooling mixing plexes of single sample puri? cation puri? cation multiplex pcr dilution universal pcr clean up mixing clean up pooling sequencing data analysis mastr plus work? ow ngs ? agilent technologies, inc. 2017 printed in belgium, december 31, 2017 5991-8991enn pr7000-1437 order information cat. no. product name reactions mr-0182.024 somatic 1 mastr plus 24 mid (molecular identifiers) kits are necessary to complete the workflow. for research use only. not for use in diagnostic procedures. multiplicom n.v. a alilelaan niel elgium .agilent.com e mtp.customerserviceagilent.com t f
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